Biologia Celular e Molecular

Abstrato

A Method of Screening for Trisomy 18 by Detecting Amniotic Fluid Punctures

Junbei Xia n g, Qian Wan

Objective: We aimed to develop a new method of screening for Trisomy 18 by detecting amniotic fluid punctures to complement the current methods.

Method: Two commercially available genomic DNA extracted from the amniotic fluid puncture of the pregnant woman with the Trisomy 18 fetus, two genomic DNAs extracted from two healthy male and four genomic DNAs extracted from four healthy female were used as the qPCR template DNAs and the commercially available Sybr green qPCR mater mix were used; we designed and synthesized 5 pairs of qPCR primers respectively corresponding to IL- 10 gene on 1# chromosome, STAT1 gene on 2# chromosome, CXCR3 gene on X chromosome, TSPY1 gene on Y chromosome and LINC01910 gene on 18# chromosome. We then performed Sybr green qPCR measurement.

Results: We processed the qPCR data by mathematical calculation and finally formed a new algorithm. Using the new algorithm, we easily distinguished the Trisomy 18 female samples out of the normal female samples.

Conclusion: We developed a new method of screening for Trisomy 18 for the female fetus by detecting amniotic fluid punctures to complement the current methods.