Ciências Marinhas: Pesquisa e Desenvolvimento

Abstrato

Effect of Liver Biotoxins of Certain Marine Fishes on Mouse Cell Culture

Yogesh Kumar and Pani Prasad Kurcheti

Biotoxins from certain marine fishes are not only harmful to marine animals and their native environment, but also have pharmacological importance, and can be used as medicines. An investigation was carried out to study the effects of liver biotoxins of marine fish (Chelonodon patoca), catfish (Osteogeneiosus militaries), and mackerel (Rastrelliger kanagurta) on mouse, and their established kidney and liver cell lines. Among the three toxins, puffer liver toxin was the most toxic in mouse, with 5 mouse units/ml, followed by 2.5 mouse units/ml and 0.67 mouse units/ml of catfish and mackerel, respectively. The morphological rigidity, viability, swelling and syncitia formation, followed by cell death, are the common features recorded in both cell lines. The Tissue Culture Infective Dose (TCID50) was the highest for puffer liver toxin in both cell lines, followed by catfish and mackerel liver toxin.