Biotecnologia e Biomateriais

Abstrato

Genome Wide Identification of FH2 Protein (Formin) Gene Family in Homo Sapiens

Hira Ashfaq

Formin (FH2) proteins are involved in the actin filament nucleation, elongation, microtubule organization, cytokinesis, regulation of cytoskeleton during cell division, and cell polarization. These genes consist of highly conserved FH2 domain which are involved in the assembly of actin filaments. In current study, a genome wide analysis of formin genes was performed in Homo sapiens by using Arabidopsis thaliana FH2 protein as a reference genome. In total, 89 members of FH2 containing formin genes were identified in H. sapiens and designated as HSF, respectively. Phylogenetic analysis clustered formin genes into seven subfamilies (INF, DRF, DIAPH, FHOD, FMN, FMNL, DAAM). Conserved domain analysis of the formin genes revealed that all the formin genes share a common FH2 domain. Phylogenetic analysis revealed that all the formin genes undergo duplication event during evolution, so show close relationship with each other. Serial cloner analysis reveals the graphical construction and restriction map of the INF gene. Current study offers enough understanding of formin genes in H. sapiens, that will be useful for additional functional analysis of these genes.