Sistema Gastrointestinal e Digestivo

Abstrato

High Mobility Group Box 1 (HMGB1) is Associated with Progression and Poor Prognosis in Pancreatic Cancer

Xu Yang Liang, Qian Qian Li, Zong Li Zhang, Chong Qi Jia, Li Li, Chun Tao Liu, Yong Mei Yang and Bao Quan Cheng

Aim: Increased expression of High Mobility Group Protein Box 1(HMGB1) has been reported with several different tumor types, but the role of HMGB1 in pancreatic cancer is not fully understood. The aim of this study is to detect the expression of HMGB1 and evaluate the role of HMGB1 in pancreatic cancer. Methods: The expression level of HMGB1 in pancreatic cancer cell line PANC-1 was detected by western blot analysis and reverse transcription PCR. Furthermore, primary tumors tissues of 35 patients with resected pancreatic cancer(T2-4 N0-2 M0-1) were stained immune histochemically using the polyclonal anti-HMGB1 antibody. The prognostic relevance of HMGB1 expression was evaluated by univariate Kaplan-Meier among 35 patients. Results: HMGB1 is over expressed in pancreatic cancer.HMGB1 overexpression was significantly associated with clinical stage (p<0.05). Univariate Cox regression showed that the survival time was significantly different between groups with high and low expression of HMGB1, indicating that high level of HMGB1correlated with a shorter survival time (P<0.05; HR, 2.53; 95%CI, 1.14-5.64). Conclusions: HMGB1 might promote pancreatic cancer progression and HMGB1 expression was an independent prognostic indicator for patients’ survival