Biologia Celular e Molecular

Abstrato

PSMC2 Knockdown Inhibits Multiple Myeloma Cell Proliferation and Enhances Apoptosis

Yuquan Ma

Proteasome 26S subunit ATPase 2 (PSMC2) has been identified as being potentially related to certain human cancers. However, the expression levels and functional importance of PSMC2 in multiple myeloma are still uncertain. PSMC2 expression in the levels of mRNA and protein was detected by qRT-PCR and western blot assay. The present study concentrated on clarifying the significance of PSMC2 on multiple myeloma cell behaviors including proliferation, migration and apoptosis by the CCK8 assay, the transwell assay and the flow cytometry. PSMC2 knockdown caused by RNA interference in multiple myeloma cell lines would significantly suppress cell proliferation, migration, enhance apoptosis and arrest cell cycle. Our results reflected that PSMC2 knockdown could inhibit multiple myeloma cell proliferation and enhance apoptosis and that the inhibition of PSMC2 might be a considerable therapeutic strategy for the treatment of multiple myeloma.