Nosso grupo organiza mais de 3.000 Séries de conferências Eventos todos os anos nos EUA, Europa e outros países. Ásia com o apoio de mais 1.000 Sociedades e publica mais de 700 Acesso aberto Periódicos que contém mais de 50.000 personalidades eminentes, cientistas de renome como membros do conselho editorial.
Periódicos de acesso aberto ganhando mais leitores e citações
700 periódicos e 15 milhões de leitores Cada periódico está obtendo mais de 25.000 leitores
Akihiro Arakawa, Nobuaki Shiraki, Tomonori Tsuyama, Shoen Kume, Daigo Iwahata and Naoyuki Yamada
Zinc, iron and copper are essential mineral nutrients for the human body to function. It is also known that there are biological processes which relate to metals in the body including cells. Therefore, understanding about intracellular metals is important for the regeneration medicine. Intracellular metals were thought to be able to be divided into two forms by function: i) protein binding metals and ii) labile metals. However, it is difficult to quantify the concentration in both intracellular metal forms. In this study, the analytical method including the pre-treatment and HPLC coupled to ICP-MS (HPLC-ICP-MS) techniques was developed. Our method is able to fractionate two forms of intracellular metals and quantify each concentration, simultaneously. Finally, the presented method was applied to analysis of several cell states. As a result, dynamic changes of intracellular metal concentration were demonstrated during cell differentiation from iPS cells to pancreatic β cells.