Biotecnologia e Biomateriais

Abstrato

T-786C Polymorphism of Endothelial Nitric Oxide Synthase Gene and Serum Level of Nitric Oxide in Nonsmoker and Nondiabetic Patients Suffering from Coronary Artery Disease

Yaghoubi AliReza, Khaki-Khatibi Fatemeh, Zarghami Nosratallah, Rahbani-Nobar Mohammad and Porhasan Mohammad

Background: Various mutations on endothelial nitric oxide synthase (eNOs) gene cause reduced production of NO, the endothelial relaxing factor, and may accelerate the process of atherosclerosis. The study designed to investigate the frequency of T-786C polymorphism of the gene in patients suffering from CAD in North West of Iran.

Material and methods: One hundred twenty subjects including 60 patients with angiographically diagnosed CAD and 60 age and sex matched CAD-free subjects as control were studied. The levels of Nitric oxide in the samples were measured with the Griess Method. The genotype studies were carried using allele specific PCR.

Results:Comparing with the control reduced levels of NO was noticed in the patient group (P<0.05) and significantly high frequency of eNOs -786C genotype was found in CAD patients (P<0.05).

Conclusions:The low levels of NO and increased frequency of T-786C polymorphism might be a risk factor in progression of coronary artery disease in the studied subjects.