Sistema Gastrointestinal e Digestivo

Abstrato

The perturbation of fatty acids of erythrocyte membranes and blood serum in patients with colorectal cancer are potential biomarkers for the diagnosis of disease

Kruchinina Margarita Vitalievna

The aim of the study was to investigate the perturbation of fatty acids (FA) of erythrocyte (RBC) phospholipid membrane and blood serum (BS) in order to find biomarkers which could be used for early screening, determination of progression. The CRC patients (63,2+9,4 years old) included 45 patients with early stage (TNM staging I-II), 55 with late stage (III-IV) and different tumour locations. RBC membrane and BS FA percentages were measured using GC/MS system triple quad Agilent 7000B (USA). A total of 21 differentially expressed FA in RBC membrane and BS were identified. Each FA class demonstrated specific changing trends in CRC progression (down-regulation in SFA, MUSA; up-regulation in PUFA during cancer progression). Panel 1 containing FA of RBC and BS: C20:2, C20:3, С20:4, С22:4, С22:5, С22:6 - achieved high diagnostic accuracy (0,79) with AUC of 0.86 (sens.0.78, specif.0.87) for differentiating early stage patients from healthy, which was better than the carcinoembryonic antigen biomarker. The model 2, including С18:1, С20:2, С20:3, С20:4, С22:4, С22:5, С22:6 - showed the best predictive power when comparing the early and late CRC stages (AUC 0.80, RR11.63). The model 3 (FA of RBC - С14:0, С16:0, С16:1, С18:1, С20:3, С20:4, С22:4, С22:5, С22:6) allowed us to determine the presence of metastases (AUC0.83, OR=2.29). Probably, FA modifications are essential for correct signalling, including Hh and Want pathways, so perturbation of FA are closely associated with carcinogenesis. Created diagnostic panels, including FA of RBS and BS, should be considered promising for screening, early diagnosis, detection of CRC progression.